Date : February 23–27, 2025
📍 Centre of Biotechnology of Sfax (CBS)
As part of the scientific capacity-building activities of the NGS-4-ECOPROD project, a hands-on workshop titled
« Metataxonomics and Functional Metagenomics from Extreme Environments for Advanced Microbial Enzyme Discovery (M2E2-ENZYMES) »
was held from February 23 to 27, 2025, at the Centre of Biotechnology of Sfax (CBS).
This intensive training session aimed to provide young researchers, PhD students, and laboratory professionals with advanced knowledge and practical skills in exploring microbial diversity and enzyme functionality using next-generation sequencing and bioinformatics approaches.
Key Highlights and Objectives

• To introduce participants to cutting-edge tools and methods in metataxonomics and functional metagenomics, particularly as applied to extreme environments
• To train attendees on experimental design, sample processing, NGS workflows, and computational analysis for enzyme discovery
• To support the exploration of novel microbial enzymes with potential applications in green biotechnology, biocatalysis, and sustainable production systems

Distinguished Participation
The workshop was honored by the presence and active contribution of Prof. Joël Lachuer, French partner of the project and expert in functional genomics and biotechnological innovation. Prof. Lachuer delivered in-depth lectures and provided hands-on mentoring during practical sessions, greatly enriching the learning experience of all participants.
Workshop Program
Over the five days, the workshop covered:

• Theoretical sessions on microbial ecology, gene mining, and enzymatic potential in extreme environments
• Wet lab practicals: DNA extraction, PCR amplification, and preparation of metagenomic libraries
• Bioinformatics modules: Quality control, taxonomic profiling (e.g., QIIME2, Kraken2), functional annotation, and enzyme prediction pipelines
• Case studies showcasing successful enzyme discovery from thermophilic and halophilic environments
• Group discussions and project-building activities to encourage collaborative research initiatives

Impact and Feedback
The M2E2-ENZYMES workshop was met with enthusiastic participation and positive feedback, reinforcing the project’s mission to enhance regional scientific competencies and promote innovation in the field of microbial biotechnology. It also opened the door to new collaborative opportunities for bioprospecting of extreme environments, particularly within the Tunisian ecosystem.
This activity falls under Work Package 2 (WP2): Microbial Resource Exploration, and is fully aligned with the strategic goals of the project in enzyme discovery, eco-innovation, and data-driven biotechnology development.
🔬 The workshop represents a key milestone in strengthening the scientific foundations necessary for the success of NGS-4-ECOPROD and its broader objectives in sustainable biotechnological advancement.